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KMID : 0613819990090010022
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Journal of Life Science
1999 Volume.9 No. 1 p.22 ~ p.28
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Isolation and Identification of Exo-Inulinase Producing Bacterium and Optimization fo the Enzyme Production
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Abstract
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A bacterium producing exo-inulinase was isolated from soil and identified Pseudomonas sp. and named as Pseudomonas sp. NO5. The optimal culture conditions for the efficient production of exo-inulinase from Pseudomonas sp. NO5 were obtained by cultivating with the medium 1% sucrose, 0.5% yeast extract, 0.5% (NH$_{4}$)$_{2}$HPO$_{4}$, 0.05% Mgso$_{4}$, 7H$_{2}$O, 0.001% and FeSO$_{4}$$\\cdot$7H$_{2}$O at 37$\circ$C in initial pH 7.0 for 20 hours. The enzyme was induced maximally in the presence of sucrose or inulin at early phase about 20 hour after cultivation.
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